MersV1, France, Analysis, bibRecord, 000319

Primer Selection by HIV-1 Reverse Transcriptase on RNA – tRNA3Lysand DNA – tRNA3LysHybrids

Identifieur interne : 000319 ( France/Analysis ); précédent : 000318; suivant : 000320

Primer Selection by HIV-1 Reverse Transcriptase on RNA – tRNA3Lysand DNA – tRNA3LysHybrids

Auteurs : Gulnara Yusupova [France] ; Jean-Marc Lanchy [France] ; Marat Yusupov [France] ; Gérard Keith [France] ; Stuart F. J. Le Grice [États-Unis] ; Chantal Ehresmann [France] ; Bernard Ehresmann [France] ; Roland Marquet [France]

Source :

Journal of Molecular Biology [ 0022-2836 ] ; 1996.

RBID : ISTEX:D8B32578E69D19A52A2692EF480D1F63B805D14A

English descriptors

KwdEn :
- HIV-1, polymerase, retrovirus, reverse transcriptase, transfer RNA.
Teeft :
- Annealed, Biol, Destefano, Ehresmann, Human virus, Human virus type, Hybrid, Isel, Last step, Marquet, Natural trnalys, Nucleic, Nucleic acids, Nucleotide, Oligomers, Polypurine tract, Primer, Primer binding site, Primer trna, Primer usage, Rnase, Transcriptase, Transcription, Trna, Trnalys, Trnalys complexes, Utrna3.

Abstract

Abstract: During reverse transcription of the genomic RNA of human immunodefi ciency virus type 1 (HIV-1) into double-stranded DNA, reverse transcriptase (RT) must accommodate RNA – RNA, DNA – RNA, RNA – DNA and DNA – DNA hybrids as primer-template. In this study, we examined extension of RNA – tRNA3Lysand DNA – tRNA3Lyscomplexes by HIV-1 RT. When the 3′ end of tRNA3Lysis annealed to oligoribonucleotides, tRNA3Lys, but not the complementary RNAs, is extended by HIV-1 RT, indicating that tRNA3Lysis efficiently used as primer and RNA as template. An opposite primer usage is observed when tRNA3Lysis annealed to complementary oligodeoxyribonucleotides. In this case, the oligodeoxyribonucleotides are efficiently used as primer and tRNA3Lysas template. This result indicates that the nature of nucleic acid bound to tRNA3Lysdetermines which strand of the RNA – tRNA3Lysand DNA – tRNA3Lyshybrids is extended by HIV-1 RT. When an oligoribonucleotide is annealed to an unmodified transcript of tRNA3Lys, both nucleic acids are extended by HIV-1 RT, indicating that specific selection of tRNA3Lysas primer requires the post-transcriptional modifications of tRNA3Lys.

Url:

https://api.istex.fr/ark:/67375/6H6-DS2C0GGF-7/fulltext.pdf

DOI: 10.1006/jmbi.1996.0463

Affiliations:

Links toward previous steps (curation, corpus...)

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Links to Exploration step

ISTEX:D8B32578E69D19A52A2692EF480D1F63B805D14A

Le document en format XML

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<author><name sortKey="Yusupova, Gulnara" sort="Yusupova, Gulnara" uniqKey="Yusupova G" first="Gulnara" last="Yusupova">Gulnara Yusupova</name>
</author>
<author><name sortKey="Lanchy, Jean Marc" sort="Lanchy, Jean Marc" uniqKey="Lanchy J" first="Jean-Marc" last="Lanchy">Jean-Marc Lanchy</name>
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<author><name sortKey="Yusupov, Marat" sort="Yusupov, Marat" uniqKey="Yusupov M" first="Marat" last="Yusupov">Marat Yusupov</name>
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<author><name sortKey="Keith, Gerard" sort="Keith, Gerard" uniqKey="Keith G" first="Gérard" last="Keith">Gérard Keith</name>
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<author><name sortKey="Le Grice, Stuart F J" sort="Le Grice, Stuart F J" uniqKey="Le Grice S" first="Stuart F. J." last="Le Grice">Stuart F. J. Le Grice</name>
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<author><name sortKey="Ehresmann, Chantal" sort="Ehresmann, Chantal" uniqKey="Ehresmann C" first="Chantal" last="Ehresmann">Chantal Ehresmann</name>
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<author><name sortKey="Ehresmann, Bernard" sort="Ehresmann, Bernard" uniqKey="Ehresmann B" first="Bernard" last="Ehresmann">Bernard Ehresmann</name>
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<author><name sortKey="Marquet, Roland" sort="Marquet, Roland" uniqKey="Marquet R" first="Roland" last="Marquet">Roland Marquet</name>
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<keywords scheme="Teeft" xml:lang="en"><term>Annealed</term>
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<front><div type="abstract" xml:lang="en">Abstract: During reverse transcription of the genomic RNA of human immunodefi ciency virus type 1 (HIV-1) into double-stranded DNA, reverse transcriptase (RT) must accommodate RNA – RNA, DNA – RNA, RNA – DNA and DNA – DNA hybrids as primer-template. In this study, we examined extension of RNA – tRNA3Lysand DNA – tRNA3Lyscomplexes by HIV-1 RT. When the 3′ end of tRNA3Lysis annealed to oligoribonucleotides, tRNA3Lys, but not the complementary RNAs, is extended by HIV-1 RT, indicating that tRNA3Lysis efficiently used as primer and RNA as template. An opposite primer usage is observed when tRNA3Lysis annealed to complementary oligodeoxyribonucleotides. In this case, the oligodeoxyribonucleotides are efficiently used as primer and tRNA3Lysas template. This result indicates that the nature of nucleic acid bound to tRNA3Lysdetermines which strand of the RNA – tRNA3Lysand DNA – tRNA3Lyshybrids is extended by HIV-1 RT. When an oligoribonucleotide is annealed to an unmodified transcript of tRNA3Lys, both nucleic acids are extended by HIV-1 RT, indicating that specific selection of tRNA3Lysas primer requires the post-transcriptional modifications of tRNA3Lys.</div>
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   |texte=   Primer Selection by HIV-1 Reverse Transcriptase on RNA – tRNA3Lysand DNA – tRNA3LysHybrids
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Primer Selection by HIV-1 Reverse Transcriptase on RNA – tRNA3Lysand DNA – tRNA3LysHybrids

Primer Selection by HIV-1 Reverse Transcriptase on RNA – tRNA3Lysand DNA – tRNA3LysHybrids

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri